Composite

Part:BBa_K4880004:Design

Designed by: Xinran Xia   Group: iGEM23_BASIS-China   (2023-08-26)


Ptrc-theo-PaFS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1071
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1220
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our design, the expression of farnesene synthase is controlled by IPTG and theophylline. Transcription is regulated by IPTG and translation initiates only when there is theophylline present.

Source

This composite part combines BBa_K3930029 and BBa_K4880000.

References